Introduction: Group D streptococci are well known to cause newborn septicemia and meningitis, but Streptococcus bovis group strains rarely cause serious neonatal infections in Korea. Central nervous system complications of neonatal S. bovis group infection have rarely been reported. In adults, Streptococcus bovis group strains are known to cause bacteremia and endocarditis, and are associated with gastrointestinal malignancy. However, there have been few reports on meningitis and septicemia in infants. This report describes a case of bacteremia and meningitis due to Streptococcus bovis (biotype II/2) with delayed CNS complication. Case report: A 28-day-old male infant was admitted with a 1-day history of fever and lethargy, and moaning sounds. A lumbar puncture was performed and cerebrospinal fluid (CSF) analysis showed marked pleocytosis with a white blood cell count of 4,000/µL (78 % neutrophils, 8 % lymphocytes), red blood cell count of 440/µL, protein concentration of 319 mg/dL, and glucose concentration of 4 mg/dL. Cultures of blood, CSF, and urine obtained by bladder puncture grew S. bovis group strains on day 5. Empirical antibiotic treatment was initiated immediately after admission with intravenous ampicillin 300 mg/kg/day and cefotaxime 200 mg/kg/day. The cultured bacteria were sensitive to penicillin, cefotaxime, and clindamycin. Amplification and sequencing of ribosomal RNA identified Streptococcus gallolyticus subsp. pasteurianus. He was discharged after 21 days of intravenous antibiotic therapy with bilateral reduction in visual evoked potentials, but no empyema or subdural effusion on cranial ultrasonography. Two weeks later, he was re-admitted with a fever and short episodes of tonic-clonic movements of the left arm and leg. Brain MRI showed marked bilateral frontal subdural effusion. Subdural fluid showed pleocytosis, but grew no pathogens. He was discharged after 31 days of antibiotic therapy, and no neurological sequelae were observed at the 9-month follow-up. Conclusion: To the best of the authors’ knowledge, this report presents the first report of neonatal S. gallolyticus subsp. pasteurianus infection causing urinary tract infection, septicemia, meningitis, and delayed central nervous system complications, emphasizing the need for physician awareness of S. bovis infection in this age group.